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Deciphering the variation of floral polymorphism in Narcissus (Amaryllidaceae)

Daniel Barranco Álvarez. University of Seville. Supervisors: Juan Arroyo, Rocío Santos Gally & Violeta Simón Porcar. Start in 2015.
Summary:
Style polymorphic Narcissus species often display spatial variation among populations in morph-ratio and perianth traits. Deterministic and stochastic processes may play a role in this variation. For example, morph-ratio variation in Narcissus may result from morph-specific differences in the rates of selfing, and assortative and dissasortative cross-pollination in populations. All of these crosses are possible because polymorphic species are characterized by the absence of an intra-morph incompatibility system and some are also self-compatible. Pollinator shifts have also been useful to understand variation in morph ratio across populations. Furthermore, historical stochastic factors (i.e. founder events, drift) can account for population structure. Detailed understanding of the maintenance of morphs requires thorough investigation across the species’ range, which is rarely achieved in widespread species. Here we analysed the influence of historical stochastic factors (phylogeography) and deterministic factors (pollinators and incompatibility systems), on the morph-ratio variation among populations of four Narcissus species (N. assoanus, N. dubius, N. rupicola and N. cuatrecasasii). The historical factors have been determined through Bayesian phylogeographic analyses. We used plastid sequences from a total of 193 populations. Pollinator environment was studied by means field observations. We determined visitation rates of pollinators in two representative populations of each species which have differences in the morph-ratio. Finally, the incompatibility system was determined from published and experimental data. This work will show the relative relevance of different factors (i.e. historical, ecological and genetic) on the stylar-polymorphism maintenance and morph-ratio variation across populations.