Structural Parameters of DNA



DNA form A (Anhydrous) B (Biological) Z (Zig-zag)
Occurrence DNA:RNA

RNA:RNA

Major form in vivo High salt form

Py-pu sequences

Helical rotation Right Right Left
Diameter (Å) 23 20-22 18
Bp/turn 11+ 10+ 12 (6 py-pu dimers)
Pitch/helical turn (Å) 28 33.2 44.6
Helical rise/bp (Å) 2.6-3.3 3.0-3.4 7.4b
Rotation per base (°) 32.7 36 -60b
Major groove (depth x width)(Å) 13.5 x 2.7 8.5 x 11.7 nil
Minor groove (depth x width)(Å) 2.8 x 11 7.5 x 5.7 Narrow and deep
Base tilt relative to helical axis (°) High,10 to 20 Low = -1.2 -7
Mean Propeller twist (°) 18 16 c.a. 0
Displacement relative to helical axis (Å) 4.4 - 4.9c ~ 0 -4
Glycosyl bond conformation relative to minor groove Anti Anti Py = anti ; pu = syn
Sugar pucker C3'-endo C2'-endo Py = C2' -endo Pu = C3' -endo
Distance between adjacent phosphates (Å) 5.9 7.0 5.7 - 7.3



a: cf Dickerson 1992 and Saenger 1984

b: Values for dinucleotide repeats

c: resulting in an open central cylinder of 3 to 5 Å